A bioreactor system for the nitrogen loop in a Controlled Ecological Life Support System.

As space missions become longer in duration, the need to recycle waste into useful compounds rises dramatically. This problem can be addressed by the development of Controlled Ecological Life Support Systems (CELSS) (i.e., Engineered Closed/Controlled Eco-Systems (ECCES)), consisting of human and plant modules. One of the waste streams leaving the human module is urine. In addition to the reclamation of water from urine, recovery of the nitrogen is important because it is an essential nutrient for the plant module. A 3-step biological process for the recycling of nitrogenous waste (urea) is proposed. A packed-bed bioreactor system for this purpose was modeled, and the issues of reaction step segregation, reactor type and volume, support particle size, and pressure drop were addressed. Based on minimization of volume, a bioreactor system consisting of a plug flow immobilized urease reactor, a completely mixed flow immobilized cell reactor to convert ammonia to nitrite, and a plug flow immobilized cell reactor to produce nitrate from nitrite is recommended. It is apparent that this 3-step bioprocess meets the requirements for space applications.     

Injuries to plants from controlled environment contaminants.

The use of controlled environments is subject to problems from contaminants emitted from materials of the system and from plants. Many contaminants are difficult to identify because injurious dosages are very low, there is a lack of information on what compounds injure plants, because species and cultivars differ greatly in their sensitivity to injury and injury symptoms often are not distinctive. Plastics have been shown to emit many different volatile compounds. The compound, di-butyl phthalate, contained in certain flexible plastics, has been shown to be very toxic to plants. Other injuries have been produced by caulking compounds and bonded screening. Paints have been shown to release xylene that is toxic to plants. Steam for humidification can cause problems because of hydroxylamines and other compounds added to steam used for heating to control fungal growth in return lines. Mercury, from broken thermometers is a particular problem in growth chambers because small quantities can collect in cracks and slowly volatilize to slow growth of plants. Plants themselves release large quantities of volatile hydrocarbons, with ethylene being the commonly recognized chemical that can be damaging when allowed to accumulate. People release large quantities of carbon dioxide which can cause variations in the rate of growth of plants. Contaminant problems can be controlled through filtering of the air or ventilation with make-up air, however the potential for problems is always present and careful testing should be undertaken with the particular species and cultivars being grown insure that there are no toxic agents altering growth in each particular controlled environment being utilized.     

Molecular alterations in tumorigenic human bronchial and breast epithelial cells induced by high LET radiation.

Carcinogenesis is a multi-stage process with sequence of genetic events governing the phenotypic expression of a series of transformation steps leading to the development of metastatic cancer. In the present study, immortalized human bronchial (BEP2D) and breast (MCF-10F) cells were irradiated with graded doses of either 150 keV/micrometer alpha particles or 1 GeV/nucleon 56Fe ions. Transformed cells developed through a series of successive steps before becoming tumorigenic in nude mice. Cell fusion studies indicated that radiation-induced tumorigenic phenotype in BEP2D cells could be completely suppressed by fusion with non-tumorigenic BEP2D cells. The differential expressions of known genes between tumorigenic bronchial and breast cells induced by alpha particles and their respective control cultures were compared using cDNA expression array. Among the 11 genes identified to be differentially expressed in BEP2D cells, three (DCC, DNA-PK and p21(CIP1)) were shown to be consistently down-regulated by 2 to 4 fold in all the 5 tumor cell lines examined. In contrast, their expressions in the fusion cell lines were comparable to control BEP2D cells. Similarly, expression levels of a series of genes were found to be altered in a step-wise manner among tumorigenic MCF-10F cells. The results are highly suggestive that functional alterations of these genes may be causally related to the carcinogenic process.     

Collisions in space: A retrospective overview of ISAS studies

A chronological review of studies in ISAS concerning collisions in space is presented. The collision probability in space with artificial orbiting bodies was estimated, and a Space Traffic Control System was proposed, in 1971. The design of a space station for safety against collision hazards was discussed in 1972. A trajectory optimization technique for low-thrust multiple rendezvous mission in order ti sweep space debris around the earth was developed in 1977. In 1984, the collision probability was reestimated using space bedris data accumulated for more than a decade. Several experimental projects in ISAS, such as hypervelocity impact experiments using a railgun system, sampling and measuring of alumina particles in exhaust plume of solid-propellant propellant rocket motors, and a result of analysis on the behavior of such alumina particles in orbit are also introduced.     

Coarse attitude determination from Earth albedo measurements

Attitude estimation algorithms for the Thrusted Vector Mission which determine attitude based on Sun sensor and very coarse albedo sensor measurements are presented. On the basis of these measurements, it has been demonstrated by comparison with more accurate gyro-based attitude that it is possible to estimate three-axis attitude with an average error per axis of 11 deg. Most of this error is about the Sun direction. Both deterministic quick-look and optimal estimates are examined     

Adaptive Forward Error Control for Digital Satellite Systems

Two hybrid schemes of time-frequency resource sharing to increase the rain margin of Ku-and Ka-band satellite systems are proposed. Scheme 1 requires sharing a small pool of bandwidth for adaptive forward error control coding, sharing a small pool of time frame for rate reduction, and sharing a portion of low frequency time-division multiple access (TDMA) back-up frame for downlink transmission to the rain affected stations. Scheme 2 utilizes variable rate modulation and forward error correction, shares a small pool of time frame for rate reduction, and shares a portion of low frequency TDMA back-up frame. Effective usable capacities of the system using these schemes are calculated. Distribution of resources in order to maximize the effective usable capacity is also analyzed. The results obtained are compared with other adaptive schemes. Preliminary analysis shows that the utilized capacity of scheme 1 exceeds 99 percent of the effective usable capacity possible if it never rains for an outage of 0.05 percent and fade margin of 2.5 dB. For scheme 2 similar performance is achievable at a fade margin of 1.5 dB. For higher outage objectives the loss of effective utilized capacity is higher for scheme 2.     

HEAO-1 and BBXRT non-source X-ray observations

A more appropriate title for this talk would have been “Measurements of Large Scale Structure from X-ray Background Fluctuations”. While it has long been recognized that the X-ray Background (XRB) is primarily of a cosmological origin (with z < a few), it has recently become apparent that surface brightness fluctuations in the surveys of the XRB can be used to trace the distribution of matter in much the same way as complete catalogs of individual objects. The distance which is probed is related to the angular resolution of the detector; for the HEAO-1 A2 experiment, which provides the best all-sky data base for the XRB in the 2–20 keV band, the effective depth is a few 100 Mpc.     

Ginga observations of Seyfert galaxies

We observed twenty-eight Seyfert 2 galaxies with the Japanese X-ray satellite, Ginga, and found Seyfert 2 galaxies, in general, have the X-ray spectral characteristics of obscured Seyfert 1 nuclei. This result agrees with the predictions from Unified Seyfert model proposed by Antonucci and Miller /1/. However, among the observed Seyfert 2 galaxies, there are a few galaxies with no evidence of an obscuration, contrary to the general predictions of the unified model. We note that type 2 AGN will contribute to the Cosmic Diffuse X-ray Background, if the unified Seyfert model can be extended to the far distant AGN such as quasars.     

Optimal multiple level decision fusion with distributed sensors

A data fusion model consisting of several levels of parallel decision fusions is considered. Global optimization of such a model is discussed to obtain the fusion rules for overall optimal performance. The reliability analysis of the proposed model is carried out to establish its superiority over the existing parallel and serial fusion models